LABORATORIES FOR CELL CULTURE AND CELL-BASED ASSAYS

LABORATORIES FOR CELL CULTURE AND CELL-BASED ASSAYS

COMT offers the possibility to use its laboratories to culture cells provided by the customer or available at COMT. Along with the Cell Culture Laboratories, the Centre offers access to a variety of primary cell cultures, mammalian cell lines and hybridomas. All cell lines used in the Centre’s Cell Culture Laboratories are assured to free of virus and bacteria. Investigators may cultivate their own cell lines and, in this case, a “Certificate of Analysis” to confirm the absence of contamination is needed.

Laboratories for Cell Culture and Cell-Based Assays are generally equipped with different optical microscopes and instruments for live-cell monitoring and for cell-based assays.

The use of some of the above, more sophisticated equipment may imply additional costs.

Capabilities of long-term storage of sensitive materials and cells are guaranteed with -80°C freezers and liquid nitrogen dewars for cryopreservation.

Contact persons:

Dr Carlotta Alias, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-906003

Dr Serena Galati, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-905608

Dr Elisa Tamburini, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.>">This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-906003

Standard fees:

35 Euro/hour – 155 Euro/day forInternal users

75 Euro/hour – 350 Euro/day for External users

Available equipment:

  • Biosafety Cabinet
  • CO2 incubators
  • Water bath with temperature control system
  • Inverted microscope
  • Bench centrifuge
  • Vortex
  • Pipettes and pipette tips and consumables

Other specific equipment:

  • Magnetic beads system for cell isolation and cell separation
  • Electroporators
  • Cell shape and vitality analysis systems
  • Bench-size bioreactors

Dedicated equipment:

  • Inverted and epi-illuminated fluorescence microscopes equipped with image analysis softwares
  • JuLI Smart Fluorescent Microscope for live video or time-lapse registration under sterile conditions
  • Fluorescence and luminescence microplate readers
  • Fluorescence/phase contrast multichannel system for cell adhesion assays under flow conditions (BioFlux)
  • Cell-cell and cell-substratum analysis system based upon impedence (xCELLigence)
  • Flow cytometers (BD Biosciences, Beckman-Coulter)