CELLULAR ASSAYS IN VITRO

CELLULAR ASSAYS IN VITRO
  1. Conventional and custom-designed in vitro cellular assays, based upon flow cytometry, real-time microscopy, laser scanning cytometry, parallel-flow assays, cell sorting, impedence measurements and multi-parametric fluorescent platforms, are offered for the analysis of cell proliferation, cytotoxicity/apoptosis, signal transduction, cell adhesion and cell migration/invasion

    • Service 1 – Flow cytometry

      Qualified flow cytometric analyses are offered on cells supplied by the customer or available at COMT. Antibodies used for the analysis can be either provided by customer or by COMT. Prices listed below do not include the cost of specific antibodies, which are charged separately if provided by COMT.

      References

      Prof. Prisco Mirandola, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-033052

      Prof. Giuliana Gobbi, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521 033142

      I) Cell immunophenotyping

      Internal price = 120 Euro/antigen/sample

      External price = 200 Euro/antigen/sample

      II) Cell viability analysis

      Internal price = 125 Euro/antigen/sample

      External price = 250 Euro/antigen/sample

      III) Cell cycle analysis

      Internal price = 125 Euro/antigen/sample

      External price = 150 Euro/antigen/sample

      IV) Immunophenotyping of platelets

      Internal price = 200 Euro/antigen/sample

      External price = 400 Euro/antigen/sample

      V) Platelets function analysis

      Internal price = 300 Euro/antigen/sample

      External price = 600 Euro/antigen/sample

    • Service 2 – Cell sorting

      Separation and enrichment of desired cell populations/cell phenotypes is offered through the MACS® Technology (magnetic nanoparticles coated with antibodies against a particular surface antigen) using the MACS ProSeparator (Miltenyi). Kits and consumables (AutoMASC columns) can be either provided by the customer or by COMT.

      References

      Prof. Prisco Mirandola, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-033052

      Prof. Giuliana Gobbi, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521 033142

      Internal price – 500 Euro/106 cells (customer-provided kit)

      External price – 800 Euro/106 cells (customer-provided kit)

      Internal price – 1.500 Euro/106 cells (kit supplied by COMT)

      External price – 2.800 Euro/106 cells (kit supplied by COMT)

    • Service 3 – Cell proliferation assays

      Accurate cell proliferation analyses on primary cell cultures or cell lines provided by the customer or proper of COMT are offered using a variety of technologies applicable to single-cell monitoring or high-throughput qualitative and quantitative screens. Employed methods include phase-contrast and fluorescence microscopy, laser scanning cytometry and impedence measurement-based systems.

      References

      Dr Carlotta Alias, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-906003

      Dr Elisa Tamburini, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-905655

      Please request quotation

    • Service 4 – Cytotoxicity/apoptosis

      Multi-parametric, highly reproducible single-cell, low- and high-throughput assessments of the agent and cell-mediated cytotoxic and pro-apoptotic activity (included autophagy) are offered using methods, cells and agents established within COMT or material provided by the customer. Assays may similarly be carried out on cell lines or primary cell cultures provided by the customer or available within COMT, are based upon the technologies reported for Cell Proliferation Assays and are complemented by Comet assays.

      References

      Prof. Annamaria Buschini, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-905608

      Dr Elisa Tamburini, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-905655

      Dr Mirca Lazzaretti, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-905608

      Please request quotation

    • Service 5 – Signal transduction

      Single-cell, low- and high-throughput analyses of signal transduction at the whole-cell level and in real-time are offered on cell lines or primary cell cultures provided by the customer or available within COMT using conventional fluorescent kits. Supportive immunochemical verification assays, involving immunohistochemistry, immunocytochemistry and Western blotting are complementary provided if requested.

      References

      Dr Elisa Tamburini, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-905655

      Dr Elena Garusi, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-906003

      Please request quotation

    • Service 6 – Cell adhesion

      Highly flexible, informative and versatile cell-substratum or cell-cell adhesion assays using cell lines or primary cells provided by the customer or available within COMT are offered at the single-cell level or in low and high-throughput settings and can be performed as pure descriptive (qualitative) or quantitative analyses under either static or dynamic (flow/shear rate-dependent) assays. Applied methods include optical and fluorescence microscopy including video time-lapse scanning laser cytometry, laser confocal microscopy, impedence based-systems and parallel-flow chamber-based microfluidity assays. Cell lines or primary cell cultures to be tested as “binders”, or substrate cells can be provided by the customer or selected by COMT. Similarly molecular substrates to be tested can be delivered by customers are provided.

      References

      Dr Carlotta Alias, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-906003

      Dr Elisa Tamburini, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-905655

      Dr Mirca Lazzaretti, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-905608

      Please request quotation

    • Service 7 – Cell migration

      End-point and real-time motile and invasive behaviour of cells is analyzed at the single-cell level, cell subsets or whole cell populations in both 2D or 3D settings. Assays are performed on both cell lines and primary cells provided by the customer or available within COMT and may involve simple molecular substrates (single components) or complex substrates (reconstituted 3D matrix, i.e. Matrigel, or assembled in vitro matrix, i.e. collagen or fibrin gel), and any conventional or specifically designed experimental condition. Assay paradigms are haptotaxis, chemotaxis and durataxis and may be combined with evaluation of mechanotransducing phenomena. Technologies involved in the performance of these assays include phase-contrast and fluorescent video time-lapse microscopy anc confocal laser microscopy, migration through porous membranes and impedence measurement-based displacement assays.

      References

      Dr Carlotta Alias, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-906003

      Dr Elena Garusi, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-906003

      Dr Mirca Lazzaretti, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-905608

      Please request quotation

It is also possible to request the preparation of extracellular matrices produced by cultured cell lines or primary cultures according to a propriety protocol developed by COMT. Cells from which deriving such matrices can be chosen among the lines available at COMT or may be provided by the customer.

Internal price - 400 Euro/matrix produced by a cell line in culture

External price - 700 Euro/matrix produced by a cell line in culture

Internal price - 750 Euro/matrix produced by primary cells in culture

External price - 1.000 Euro/ matrix produced by primary cells in culture